Methods in Structural Chemistry

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About the author:
Andreas Hofmann is the Structural Chemistry Program Leader at Griffith University's Eskitis Institute in Brisbane, Australia, and holds a Senior Honorary Research Fellow at the Faculty of Veterinary and Agricultural Sciences at the University of Melbourne. His research focuses on the structure and function of proteins in infectious and neurodegenerative diseases with a special interest in protein crystallography and biophysical methods. Among other prizes, he won the EULAR Young Investigator Award in 2004, and holds a Fellowship by the Higher Education Academy (UK).

Methods in Structural Chemistry
 

A Lab Manual

Authored by Andreas Hofmann
Edition: 3

This Lab Manual provides the experimental procedures as well as the fundamental background for methods used in a structural biochemistry laboratory. It is a collection of methods and procedures routinely used in our laboratory and subject to annual additions. This Manual is an integral part of our laboratory and as such constitutes a write-up of standard operating procedures for reference and training purposes, as well as Health & Safety and Good Laboratory Practice.

Table of Contents

0 General Data
0.1 Periodic table of the elements
0.2 Units and constants
0.3 Conversion factors
0.4 Conversion and other useful formulae
0.5 Commonly used pH-buffers
0.6 Common concentrations of selected acids and bases
0.7 Molecular mass of amino acids
0.8 Nucleotide physical properties
0.9 Genetic code
0.10 Endonuclease cleavage close to the end of DNA fragments
0.11 Multi-well matrices
1 Typical Workflows1
1.1 Bacterial expression of soluble recombinant proteins
1.2 Protein crystallisation
1.3 Protein crystal space group determination
2 Methods in Molecular Biology
2.1 Standard methods for work with bacteria
2.2 Standard methods for work with yeast
2.3 Standard methods for work with DNA
3 Methods in Protein Biochemistry
3.1 Protein Expression
3.2 Characterisation of Proteins
3.3 Chromatography
3.4 Centrifugation
4 Assays
4.1 Liposome assays
4.2 Stability measurements
4.3 Protein ligand binding by differential scanning fluorimetry
4.4 Heparin binding assay
4.5 Enzyme kinetics by stopped flow experiments
4.6 Enzyme inhibition
5 Circular dichroism spectroscopy
5.1 Introduction
5.2 Sample requirements
5.3 Buffer suitability
5.4 Data analysis
6 Monolayer Adsorption Experiments
6.1 Langmuir surface film balance NIMA Model 301A
7 Protein Crystallography
7.1 Crystallisation
7.2 X-ray diffraction
8 Data analysis and visualisation
8.1 Data analysis
8.2 Software
8.3 Independent and technical repeats
8.4 Data visualisation
8.5 Data fitting
8.6 Correlation
9 References
10 Index


Publication Date:
2015-12-31
ISBN/EAN13:
0994201036 / 9780994201034
Page Count:
120
Binding Type:
US Trade Paper
Trim Size:
6" x 9"
Language:
English
Color:
Full Color
Related Categories:
Science / Life Sciences / Biochemistry




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